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Image Search Results
Journal: Cancer cell
Article Title: Removal of N-linked glycosylation enhances PD-L1 detection and predicts anti-PD-1/PD-L1 therapeutic efficacy
doi: 10.1016/j.ccell.2019.06.008
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies PD-L1 antibody (clone 28-8 mAb) Abcam Cat#ab205921; RRID:AB_2687878 CD274 rabbit polyclonal antibody Abcam Cat#ab58810; RRID:AB_940872 PD-L1 (E1L3N) XP rabbit antibody Cell Signaling Technology Cat#13684; RRID:AB_2687655 Atezolizumab MDACC N/A α-tubulin antibody (monoclonal) Sigma-Aldrich Cat#T5168; RRID:AB_477579 Actin antibody Sigma-Aldrich Cat#A2066; RRID:AB_476693 Peroxidase-AffiniPure Goat anti-Mouse IgG (H+L) Jackson ImmunoResearch Cat#115-035-003; RRID:AB_10015289 Peroxidase-AffiniPure Goat anti-Rabbit IgG (H+L) Jackson ImmunoResearch Cat#111-035-003; RRID:AB_2313567 Goat Anti-Rabbit IgG (H+L) antibody, Fluorescein Conjugated Thermo Fisher Scientific Cat#F2765; RRID:AB_10562896 Bacterial and Virus Strains N/A Biological Samples Multi-organ carcinoma tissue array Biomax Cat# BC000119 Lung cancer tissue array Biomax Cat#NSC151 Rectal cancer tissue array This paper N/A Human pre-immunotherapy archived tissue samples This paper N/A Chemicals, Peptides, and Recombinant Proteins PNGase F New England BioLabs Cat#P0704 AEC chromogen substrate (3-amino-9-ethylcarbazole) Sigma-Aldrich Cat#A5754 N,N -dimethylformamide Sigma-Aldrich Cat#D4254 Aqua-mount mounting medium DAKO Cat#S1964 ProLong™ Gold Antifade Mountant with DAPI Invitrogen Cat# P36931 TMB Substrate Cell Signaling Technology Cat#7004 TMB High Sensitivity Substrate Solution BioLegend Cat#421501 STOP Solution Cell Signaling Technology Cat#7002 Stop Solution for TMB Substrate BioLegend Cat#423001 BSA blocking solution for ELISA Santa Cruz Biotechnology Cat#293965 Animal-Free Blocking Solution Cell Signaling Technology Cat#15019 Concanavalin A, HRP-conjugated Sigma-Aldrich Cat#L6397 Critical Commercial Assays N/A Deposited Data N/A Experimental Models: Cell Lines BT-549 ATCC ATCC#HTB-122 BT-20 ATCC ATCC#HTB-19 MDA-MB-231 ATCC ATCC#HTB-26 MCF-7 ATCC ATCC#HTB-22 H1437 ATCC ATCC#CRL-5872 A549 ATCC ATCC#CCL-185 Calu3 ATCC ATCC#HTB-55 H1299 ATCC ATCC#CRL-5803 H1355 ATCC
Techniques: Recombinant, Blocking Assay, Enzyme-linked Immunosorbent Assay, Software